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normal human colon epithelial cell model  (ATCC)


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    ATCC normal human colon epithelial cell model
    Cytotoxic response and intracellular ROS levels induced by the multimodal hydrogel nanoplatform in CRC and normal colon cell models. (A) Dose–response curve of free 5-FU in HCT-116 cells after 48 h, determined by MTT assay and used to define the experimental IC₅₀. Cell viability was normalized to the Ctrl. For visualization on a logarithmic x-axis, the Ctrl condition is plotted at a nominal concentration of 0.1 µg mL⁻¹. (B–D) MTT-based cell viability after 48 h treatment in (B) HCT-116, (C) SW480, and (D) normal human colon <t>epithelial</t> NCM460 cells. Treatment groups include Ctrl, free 5-FU (5FU), non-targeted nanoparticles (NP), HA-targeted nanoparticles (tNP), blank hydrogel (Gel), hydrogel-loaded targeted nanoparticles (Gel-tNP), and Gel-tNP combined with near-infrared irradiation (NIR, 808 nm), X-ray irradiation (XR, 2 Gy), or both. All treatments were administered at an equivalent 5-FU concentration corresponding to the IC₅₀ determined in panel (A). (E) Intracellular ROS levels in HCT-116 cells measured after 48 h using CellROX™ Deep Red and expressed as fold change relative to Ctrl. Data are mean ± SD ( n = 3). Statistical significance was assessed by one-way ANOVA with multiple-comparisons post-hoc testing. Asterisks indicate comparisons vs. Ctrl (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Brackets indicate the specific intergroup comparisons shown (e.g., dual-modal vs. tri-modal where indicated). “ns” denotes not significant
    Normal Human Colon Epithelial Cell Model, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 2012 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human colon epithelial cell model/product/ATCC
    Average 98 stars, based on 2012 article reviews
    normal human colon epithelial cell model - by Bioz Stars, 2026-05
    98/100 stars

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    1) Product Images from "Hydrogel-mediated tri-modal nanoplatform for localized colorectal cancer therapy via smart chemo–photothermal–radiotherapy"

    Article Title: Hydrogel-mediated tri-modal nanoplatform for localized colorectal cancer therapy via smart chemo–photothermal–radiotherapy

    Journal: Journal of Biological Engineering

    doi: 10.1186/s13036-026-00633-0

    Cytotoxic response and intracellular ROS levels induced by the multimodal hydrogel nanoplatform in CRC and normal colon cell models. (A) Dose–response curve of free 5-FU in HCT-116 cells after 48 h, determined by MTT assay and used to define the experimental IC₅₀. Cell viability was normalized to the Ctrl. For visualization on a logarithmic x-axis, the Ctrl condition is plotted at a nominal concentration of 0.1 µg mL⁻¹. (B–D) MTT-based cell viability after 48 h treatment in (B) HCT-116, (C) SW480, and (D) normal human colon epithelial NCM460 cells. Treatment groups include Ctrl, free 5-FU (5FU), non-targeted nanoparticles (NP), HA-targeted nanoparticles (tNP), blank hydrogel (Gel), hydrogel-loaded targeted nanoparticles (Gel-tNP), and Gel-tNP combined with near-infrared irradiation (NIR, 808 nm), X-ray irradiation (XR, 2 Gy), or both. All treatments were administered at an equivalent 5-FU concentration corresponding to the IC₅₀ determined in panel (A). (E) Intracellular ROS levels in HCT-116 cells measured after 48 h using CellROX™ Deep Red and expressed as fold change relative to Ctrl. Data are mean ± SD ( n = 3). Statistical significance was assessed by one-way ANOVA with multiple-comparisons post-hoc testing. Asterisks indicate comparisons vs. Ctrl (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Brackets indicate the specific intergroup comparisons shown (e.g., dual-modal vs. tri-modal where indicated). “ns” denotes not significant
    Figure Legend Snippet: Cytotoxic response and intracellular ROS levels induced by the multimodal hydrogel nanoplatform in CRC and normal colon cell models. (A) Dose–response curve of free 5-FU in HCT-116 cells after 48 h, determined by MTT assay and used to define the experimental IC₅₀. Cell viability was normalized to the Ctrl. For visualization on a logarithmic x-axis, the Ctrl condition is plotted at a nominal concentration of 0.1 µg mL⁻¹. (B–D) MTT-based cell viability after 48 h treatment in (B) HCT-116, (C) SW480, and (D) normal human colon epithelial NCM460 cells. Treatment groups include Ctrl, free 5-FU (5FU), non-targeted nanoparticles (NP), HA-targeted nanoparticles (tNP), blank hydrogel (Gel), hydrogel-loaded targeted nanoparticles (Gel-tNP), and Gel-tNP combined with near-infrared irradiation (NIR, 808 nm), X-ray irradiation (XR, 2 Gy), or both. All treatments were administered at an equivalent 5-FU concentration corresponding to the IC₅₀ determined in panel (A). (E) Intracellular ROS levels in HCT-116 cells measured after 48 h using CellROX™ Deep Red and expressed as fold change relative to Ctrl. Data are mean ± SD ( n = 3). Statistical significance was assessed by one-way ANOVA with multiple-comparisons post-hoc testing. Asterisks indicate comparisons vs. Ctrl (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Brackets indicate the specific intergroup comparisons shown (e.g., dual-modal vs. tri-modal where indicated). “ns” denotes not significant

    Techniques Used: MTT Assay, Concentration Assay, Irradiation



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    normal human colon epithelial cell model  (ATCC)
    98
    ATCC normal human colon epithelial cell model
    Cytotoxic response and intracellular ROS levels induced by the multimodal hydrogel nanoplatform in CRC and normal colon cell models. (A) Dose–response curve of free 5-FU in HCT-116 cells after 48 h, determined by MTT assay and used to define the experimental IC₅₀. Cell viability was normalized to the Ctrl. For visualization on a logarithmic x-axis, the Ctrl condition is plotted at a nominal concentration of 0.1 µg mL⁻¹. (B–D) MTT-based cell viability after 48 h treatment in (B) HCT-116, (C) SW480, and (D) normal human colon <t>epithelial</t> NCM460 cells. Treatment groups include Ctrl, free 5-FU (5FU), non-targeted nanoparticles (NP), HA-targeted nanoparticles (tNP), blank hydrogel (Gel), hydrogel-loaded targeted nanoparticles (Gel-tNP), and Gel-tNP combined with near-infrared irradiation (NIR, 808 nm), X-ray irradiation (XR, 2 Gy), or both. All treatments were administered at an equivalent 5-FU concentration corresponding to the IC₅₀ determined in panel (A). (E) Intracellular ROS levels in HCT-116 cells measured after 48 h using CellROX™ Deep Red and expressed as fold change relative to Ctrl. Data are mean ± SD ( n = 3). Statistical significance was assessed by one-way ANOVA with multiple-comparisons post-hoc testing. Asterisks indicate comparisons vs. Ctrl (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Brackets indicate the specific intergroup comparisons shown (e.g., dual-modal vs. tri-modal where indicated). “ns” denotes not significant
    Normal Human Colon Epithelial Cell Model, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human colon epithelial cell model/product/ATCC
    Average 98 stars, based on 1 article reviews
    normal human colon epithelial cell model - by Bioz Stars, 2026-05
    98/100 stars
    		  Buy from Supplier
    models human normal colon epithelial cells ccd841  (ATCC)
    96
    ATCC models human normal colon epithelial cells ccd841
    Cytotoxic response and intracellular ROS levels induced by the multimodal hydrogel nanoplatform in CRC and normal colon cell models. (A) Dose–response curve of free 5-FU in HCT-116 cells after 48 h, determined by MTT assay and used to define the experimental IC₅₀. Cell viability was normalized to the Ctrl. For visualization on a logarithmic x-axis, the Ctrl condition is plotted at a nominal concentration of 0.1 µg mL⁻¹. (B–D) MTT-based cell viability after 48 h treatment in (B) HCT-116, (C) SW480, and (D) normal human colon <t>epithelial</t> NCM460 cells. Treatment groups include Ctrl, free 5-FU (5FU), non-targeted nanoparticles (NP), HA-targeted nanoparticles (tNP), blank hydrogel (Gel), hydrogel-loaded targeted nanoparticles (Gel-tNP), and Gel-tNP combined with near-infrared irradiation (NIR, 808 nm), X-ray irradiation (XR, 2 Gy), or both. All treatments were administered at an equivalent 5-FU concentration corresponding to the IC₅₀ determined in panel (A). (E) Intracellular ROS levels in HCT-116 cells measured after 48 h using CellROX™ Deep Red and expressed as fold change relative to Ctrl. Data are mean ± SD ( n = 3). Statistical significance was assessed by one-way ANOVA with multiple-comparisons post-hoc testing. Asterisks indicate comparisons vs. Ctrl (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Brackets indicate the specific intergroup comparisons shown (e.g., dual-modal vs. tri-modal where indicated). “ns” denotes not significant
    Models Human Normal Colon Epithelial Cells Ccd841, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/models human normal colon epithelial cells ccd841/product/ATCC
    Average 96 stars, based on 1 article reviews
    models human normal colon epithelial cells ccd841 - by Bioz Stars, 2026-05
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Cytotoxic response and intracellular ROS levels induced by the multimodal hydrogel nanoplatform in CRC and normal colon cell models. (A) Dose–response curve of free 5-FU in HCT-116 cells after 48 h, determined by MTT assay and used to define the experimental IC₅₀. Cell viability was normalized to the Ctrl. For visualization on a logarithmic x-axis, the Ctrl condition is plotted at a nominal concentration of 0.1 µg mL⁻¹. (B–D) MTT-based cell viability after 48 h treatment in (B) HCT-116, (C) SW480, and (D) normal human colon epithelial NCM460 cells. Treatment groups include Ctrl, free 5-FU (5FU), non-targeted nanoparticles (NP), HA-targeted nanoparticles (tNP), blank hydrogel (Gel), hydrogel-loaded targeted nanoparticles (Gel-tNP), and Gel-tNP combined with near-infrared irradiation (NIR, 808 nm), X-ray irradiation (XR, 2 Gy), or both. All treatments were administered at an equivalent 5-FU concentration corresponding to the IC₅₀ determined in panel (A). (E) Intracellular ROS levels in HCT-116 cells measured after 48 h using CellROX™ Deep Red and expressed as fold change relative to Ctrl. Data are mean ± SD ( n = 3). Statistical significance was assessed by one-way ANOVA with multiple-comparisons post-hoc testing. Asterisks indicate comparisons vs. Ctrl (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Brackets indicate the specific intergroup comparisons shown (e.g., dual-modal vs. tri-modal where indicated). “ns” denotes not significant

    Journal: Journal of Biological Engineering

    Article Title: Hydrogel-mediated tri-modal nanoplatform for localized colorectal cancer therapy via smart chemo–photothermal–radiotherapy

    doi: 10.1186/s13036-026-00633-0

    Figure Lengend Snippet: Cytotoxic response and intracellular ROS levels induced by the multimodal hydrogel nanoplatform in CRC and normal colon cell models. (A) Dose–response curve of free 5-FU in HCT-116 cells after 48 h, determined by MTT assay and used to define the experimental IC₅₀. Cell viability was normalized to the Ctrl. For visualization on a logarithmic x-axis, the Ctrl condition is plotted at a nominal concentration of 0.1 µg mL⁻¹. (B–D) MTT-based cell viability after 48 h treatment in (B) HCT-116, (C) SW480, and (D) normal human colon epithelial NCM460 cells. Treatment groups include Ctrl, free 5-FU (5FU), non-targeted nanoparticles (NP), HA-targeted nanoparticles (tNP), blank hydrogel (Gel), hydrogel-loaded targeted nanoparticles (Gel-tNP), and Gel-tNP combined with near-infrared irradiation (NIR, 808 nm), X-ray irradiation (XR, 2 Gy), or both. All treatments were administered at an equivalent 5-FU concentration corresponding to the IC₅₀ determined in panel (A). (E) Intracellular ROS levels in HCT-116 cells measured after 48 h using CellROX™ Deep Red and expressed as fold change relative to Ctrl. Data are mean ± SD ( n = 3). Statistical significance was assessed by one-way ANOVA with multiple-comparisons post-hoc testing. Asterisks indicate comparisons vs. Ctrl (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Brackets indicate the specific intergroup comparisons shown (e.g., dual-modal vs. tri-modal where indicated). “ns” denotes not significant

    Article Snippet: The cytotoxicity of all treatment groups was evaluated using an MTT assay in two CRC cell lines and one normal human colon epithelial cell model. HCT-116 human colorectal carcinoma cells (ATCC ® CCL-247TM, American Type Culture Collection, USA) and SW480 human colorectal adenocarcinoma cells (ATCC ® CCL-228TM, American Type Culture Collection, USA) were used as CRC models, and normal human colon epithelial cells NCM460 (NCM460DTM; INCELL Corporation LLC, San Antonio, TX, USA) were used to provide an initial in-vitro assessment of relative tolerance in non-malignant colon epithelium.

    Techniques: MTT Assay, Concentration Assay, Irradiation